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Kvantovaya Elektronika, 2009, Volume 39, Number 3, Pages 273–278 (Mi qe13953)  

This article is cited in 2 scientific papers (total in 2 papers)

LASER BIOLOGY AND MEDICINE

Application of laser fluorimetry for determining the influence of a single amino-acid substitution on the individual photophysical parameters of a fluorescent form of a fluorescent protein mRFP1

A. A. Banishev, E. P. Vrzheshch, E. A. Shirshin

Faculty of Physics, Lomonosov Moscow State University

Abstract: Individual photophysical parameters of the chromophore of a fluorescent protein mRFP1 and its two mutants (amino-acid substitution at position 66 — mRFP1/Q66C and mRFP1/Q66S proteins) are determined. For this purpose, apart from conventional methods of fluorimetry and spectrophotometry, nonlinear laser fluorimetry is used. It is shown that the individual extinction coefficients of the chromophore of proteins correlate (correlation coefficient above 0.9) with the volume of the substituted amino-acid residue at position 66 (similar to the positions of the absorption, fluorescence excitation and emission maxima).

Full text: PDF file (164 kB)

English version:
Quantum Electronics, 2009, 39:3, 273–278

Bibliographic databases:

PACS: 87.14.E, 87.64.kv
Received: 07.08.2008

Citation: A. A. Banishev, E. P. Vrzheshch, E. A. Shirshin, “Application of laser fluorimetry for determining the influence of a single amino-acid substitution on the individual photophysical parameters of a fluorescent form of a fluorescent protein mRFP1”, Kvantovaya Elektronika, 39:3 (2009), 273–278 [Quantum Electron., 39:3 (2009), 273–278]

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  • http://mi.mathnet.ru/eng/qe13953
  • http://mi.mathnet.ru/eng/qe/v39/i3/p273

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    Citing articles on Google Scholar: Russian citations, English citations
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    This publication is cited in the following articles:
    1. Alexandr A. Banishev, Evgeny A. Shirshin, Victor V. Fadeev, Appl. Opt, 49:34 (2010), 6637  crossref  isi  scopus
    2. I.L.. Volkov, P.Y.u. Serdobintsev, A.I.. Kononov, J. Phys. Chem. C, 2013, 1311011503  crossref  isi  scopus
  • Квантовая электроника Quantum Electronics
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