Influence of femtosecond laser radiation on cells of the transplantable tumour Krebs-2
Yu. P. Meshalkinab, N. A. Popovabc, V. P. Nikolinc, V. I. Kaledinc, A. V. Kirpichnikova, E. V. Pestryakova
a Institute of Laser Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk
b Novosibirsk State Technical University
c Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk
The influence of femtosecond radiation of a titaniumsapphire laser on cells of the transplantable ascitic tumour Krebs-2 was studied. After in vitro irradiation by the pulsed fundamentalharmonic radiation with the wavelength 800 nm, pulse duration 30 fs, repetition rate 1 kHz, mean power 100 and 300 mW and exposure time 3 min, as well as by the second-harmonic radiation (40 nm, 50 fs, 120 mW), all cells were diffusely stained by the vital stain trypan blue, which may be an evidence of their death or abnormalities of membrane permeability. However, implantation of such cells to experimental animals led to formation of tumours at the transplantation site with the kinetics slightly different from the control one. In the group of mice to which the cells were inoculated after irradiation with second harmonic pulses of titanium-sapphire laser the inhibition of tumour growth was observed due to partial death of cells under the action of UV spectral components. To explain the mechanism of the observed phenomenon the possibility of pore formation (photoporation) in the cell membrane, described earlier in the papers on foreign DNA transfection into cells, is considered.
titanium-sapphire laser, femtosecond radiation, Krebs-2 tumour cells, trypan blue, photoporation, in vivo kinetics of tumour growth.
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Quantum Electronics, 2012, 42:6, 505–508
42.62.Be, 87.50.W-, 87.19.xj, 42.65.Re
Yu. P. Meshalkin, N. A. Popova, V. P. Nikolin, V. I. Kaledin, A. V. Kirpichnikov, E. V. Pestryakov, “Influence of femtosecond laser radiation on cells of the transplantable tumour Krebs-2”, Kvantovaya Elektronika, 42:6 (2012), 505–508 [Quantum Electron., 42:6 (2012), 505–508]
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