Color models of interference images of thin stratified objects in optical microscopy

Algorithms for the analysis of polychromatic interference patterns in images of thin stratified objects in optical microscopy are considered. The algorithms allow one to measure the thin-film optical thickness. A measurement method based on the comparison of colors of the interference image under study and a numerically simulated image is discussed. We discuss a mathematical model for the calculation and numerical simulation of interference patterns and algorithms for interference pattern processing. Color comparison in an RGB color model is described and limitations of such a method are shown. The feasibility of using a Lab color model is shown and algorithms of interference color comparison in this model are presented. Results of application of the presented algorithms to measuring the optical thickness of red blood cells in a blood smear are discussed. The estimation of the error and robustness of the proposed algorithms is conducted.