High-resolution Raman scattering in oligonucleotides

The high-resolution spectra of short single-stranded oligonucleotides have been measured by the high-sensitivity nonresonant Raman scattering method using the example of the system $d$(20$G$, 20$T$), where $G$ is guanine and $T$ is thymine. The samples under study have been prepared by the chemical synthesis using the solid-phase amidophosphite method on an automatic synthesizer. It has been shown that the spectra observed in the frequency range of 500–1500 cm$^{-1}$ can be sufficient for the resolution of spectral components corresponding to vibrations of individual oligonucleotide molecules, which contain a large number of atoms in the unit cell.