Magnetic particles as a carrier to select aptamer for survivin (BIRC5), a low molecular weight protein tumor marker

Magnetic iron oxide nanoparticles modified with aminosiloxane ($\gamma$-Fe$_2$O$_3$@SiO$_2$-NH$_2$) were used as a carrier to select DNA aptamers for a small protein tumor biomarker survivin (BIRC5, 16.5 kDa) from a randomized synthetic DNA library by the SELEX method. Survivin was covalently immobilized on the nanoparticles’ surface. The library enrichment was monitored by solid-phase bioluminescent immunoassay and the DNA melting method. The prospects are outlined for the use of the obtained nanoparticles for the effective selection of aptamers specific to a given target.