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Journal of Siberian Federal University. Mathematics & Physics, 2024, Volume 17, Issue 6, Pages 791–797 (Mi jsfu1212)  
Design of aptamer sandwich pairs for colorimetric detection of TNFа, Interleukin-17a and Dickkopf-1 proteins associated with ankylosing spondylitis

Elizaveta A. Shatunovaa, Anna S. Davydovaa, Nikolay A. Danilina, Arseniy A. Golyshkina, Maksim A. Korolevab, Mariya A. Vorobyevaa

a Institute of Chemical Biology and Fundamental Medicine SD RAS, Novosibirsk, Russian Federation
b Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center of Cytology and Genetics SD RAS, Novosibirsk, Russian Federation
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Abstract: We employed DNA and 2'-fluoro RNA aptamers to assembly novel systems for colorimetric microplate detection of TNFa, interleukin-17A and Dickkopf-1 proteins as biomarkers associated with ankylosing spondylitis. For each protein, we chose an optimal sandwich pair made of plate-immobilized capture aptamer and biotinylated reporter aptamer recruiting streptavidin-peroxidase conjugate. A feasibility of these test systems was demonstrated in model protein solutions.
Keywords: oligonucleotide aptamers, biosensors, colorimetric detection, ankylosing spondylitis.
Funding agency Grant number
Russian Science Foundation 22-15-20050
Government of the Novosibirsk region r-2
The study was supported by Russian Scientific Foundation (grant no. 22-15-20050) and the Government of the Novosibirsk region (agreement no. r-2).
Received: 10.06.2024
Received in revised form: 15.07.2024
Accepted: 24.09.2024
Bibliographic databases:
Document Type: Article
UDC: 577.113.7
Language: English
Citation: Elizaveta A. Shatunova, Anna S. Davydova, Nikolay A. Danilin, Arseniy A. Golyshkin, Maksim A. Korolev, Mariya A. Vorobyeva, “Design of aptamer sandwich pairs for colorimetric detection of TNFа, Interleukin-17a and Dickkopf-1 proteins associated with ankylosing spondylitis”, J. Sib. Fed. Univ. Math. Phys., 17:6 (2024), 791–797
Citation in format AMSBIB
\Bibitem{ShaDavDan24}
\by Elizaveta~A.~Shatunova, Anna~S.~Davydova, Nikolay~A.~Danilin, Arseniy~A.~Golyshkin, Maksim~A.~Korolev, Mariya~A.~Vorobyeva
\paper Design of aptamer sandwich pairs for colorimetric detection of TNFа, Interleukin-17a and Dickkopf-1 proteins associated with ankylosing spondylitis
\jour J. Sib. Fed. Univ. Math. Phys.
\yr 2024
\vol 17
\issue 6
\pages 791--797
\mathnet{http://mi.mathnet.ru/jsfu1212}
\edn{https://elibrary.ru/VKMXNM}
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