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XXVII International Symposium "Nanophysics and Nanoelectronics" N. Novgorod, March 13-16, 2023
Photonics
Study of histological sections of muscle tissues using X-ray fluorescence and Raman spectromicroscopy
I. A. Artyukova, G. P. Arutunovb, D. O. Dragunovb, N. N. Mel'nika, D. J. A. Panekea, E. V. Perevedentsevaa, A. V. Sokolovab, V. V. Sokolovaa a P. N. Lebedev Physical Institute, Russian Academy of Sciences, Moscow
b Russian National Research Medical University named after N. I. Pirogov, 117997 Moscow, Russia
Abstract:
The paper presents the results of a study of the chemical composition of myocardial tissues and striated muscles at the cellular level using a combination of X-ray fluorescence microscopy and Raman microspectrosopy. The distribution of inorganic materials (Na, Si, Ca, etc.) was measured with the help of X-ray fluorescence imaging and microanalysis workstations at ELETTRA synchrotron (Italy). Methods of infrared Raman spectromicroscopy were applied for the identification of organic compounds (lipids, amides, and polysaccharides). All experiments were carried out with unstained paraffin-embedded histological sections. The research was related to the study of sodium deposits in muscle tissues.
Keywords:
X-ray microscopy, Raman spectroscopy, histological sections, chemical fixation, sodium deposits.
Received: 07.04.2023 Revised: 07.04.2023 Accepted: 07.04.2023
Citation:
I. A. Artyukov, G. P. Arutunov, D. O. Dragunov, N. N. Mel'nik, D. J. A. Paneke, E. V. Perevedentseva, A. V. Sokolova, V. V. Sokolova, “Study of histological sections of muscle tissues using X-ray fluorescence and Raman spectromicroscopy”, Zhurnal Tekhnicheskoi Fiziki, 93:7 (2023), 953–958
Linking options:
https://www.mathnet.ru/eng/jtf7033 https://www.mathnet.ru/eng/jtf/v93/i7/p953
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