High-resolution mass spectra of biotinylated, HEG-spacered molecular probes with oligosaccharide fragments of the capsular polysaccharides from Streptococcus pneumoniae

High-resolution electrospray mass spectra (MS and MS/MS) of biotinylated, hexaethylene glycol (HEG) spacered molecular probes bearing immunogenic oligosaccharides, fragments of capsular polysaccharides of Streptococcus pneumoniae, were interpreted (four examples). Characteristic fragmentation of the aglycone at the end of the HEG chain distant from the biotin fragment has been revealed.