Abstract:
We demonstrated covalent cross-linking of two or three protein molecules specifically at the C-terminus by sortase-catalyzed conjugation of recombinant proteins with conformationally rigid-flexible tetraantennary templates, where structure of the antenna is [Gly-Gly-(N-CH2COOH)Gly]nCH2–.
Keywords:
bioconjugation, sortase, protein A, oligoglycine, protein homooligomerization.
Citation:
T. D. Melikhova, N. V. Bovin, A. D. Antipov, M. N. Tereshin, R. H. Ziganshin, A. B. Tuzikov, “Sortase-promoted synthesis of homooligomers from a monomeric protein”, Mendeleev Commun., 33:5 (2023), 624–626
Linking options:
https://www.mathnet.ru/eng/mendc476
https://www.mathnet.ru/eng/mendc/v33/i5/p624
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