SEM and SAXS characterization of recombinant lipase immobilized by adsorption on the surface of cellular carbon foam

Using modern scanning electron microscopy (SEM), a research was conducted on the supramolecular aggregation of protein globules of recombinant Thermomyces lanuginosus lipase (TLL) adsorbed on the surface of cellular carbon foam (CCF) by the two methods of physical adsorption: spontaneous and forced (impregnation–drying). After the spontaneous adsorption, more than half of the entire surface of CCF was uniformly occupied by 10–50 nm nanoparticles, which corresponded in size to the monomers and dimers of TLL globules. After the forced adsorption, protein films were formed between and around pyrolytic carbon deposits protruding beyond these films.