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Optical sensors and transducers
Chemiluminescence of a functionalized graphene surface
A. Yu. Plekhanovab, M. V. Puzykc, A. S. Usikovde, A. D. Roenkovd, A. A. Lebedeve, S. P. Lebedevf, S. A. Klotchenkof, A. V. Vasinf, Yu. N. Makarovd a The Petersburg Nuclear Physics Institute, The National Research Center "Kurchatov Institute"
b Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg
c Herzen State Pedagogical University of Russia, St. Petersburg
d Nitride Crystals Group, St.-Petersburg
e Ioffe Institute, St. Petersburg
f Peter the Great St. Petersburg Polytechnic University
Abstract:
To analyze the modification of the functionalized surface of graphene by protein molecules, a chemiluminescent enzyme immunoassay method was proposed. Using the example of functionalized graphene (FG) purification, the possibilities of chemiluminescent control of the state of its surface are shown. Methods for purifying FG from protein molecules with the restoration of the ability to resorb protein molecules are discussed. It has been shown that the FG surface can be cleaned from sorbed proteins, and a biosensor can be designed again based on such purified graphene, including with a different specificity. Therefore, the graphene sensor can be used repeatedly.
Keywords:
functionalized graphene, 1-pyrenemethylamine hydrochloride, cyclic voltammetry, chemiluminescence, monoclonal antibodies, biosensors.
Received: 28.04.2022 Revised: 03.06.2022 Accepted: 16.06.2022
Citation:
A. Yu. Plekhanov, M. V. Puzyk, A. S. Usikov, A. D. Roenkov, A. A. Lebedev, S. P. Lebedev, S. A. Klotchenko, A. V. Vasin, Yu. N. Makarov, “Chemiluminescence of a functionalized graphene surface”, Optics and Spectroscopy, 130:9 (2022), 1417–1422
Linking options:
https://www.mathnet.ru/eng/os1837 https://www.mathnet.ru/eng/os/v130/i9/p1417
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