Synthetic modulator of chymotrypsin activity based on p-tert-butylthiacalix[4]arene

A p-tert-butylthiacalix[4]arene derivative containing tryptophan moieties on the lower rim was synthesized and proposed as a chymotrypsin inhibitor. Its ability to competitively inhibit the enzyme was revealed by kinetic methods. A mechanism of the inhibitory action has been established, which consists in the binding of macrocycle tryptophan moieties to the enzyme active site, resulting in its blocking.