Properties of core–shell nanoparticles based on PLGA and human serum albumin prepared by different methods

Core–shell nanoparticles with poly(lactide-co-glycolide) cores and human serum albumin shells (PLGA/HSA NPs) were prepared by three methods: by interfacial embedding of HSA in the PLGA NP surface during their formation and by adsorption or conjugation of HSA on pre-prepared PLGA NPs. All methods yielded PLGA/HSA NPs with a size of 130–150 nm and a narrow size distribution. In vitro fluorescence microscopy revealed the integrity of all core–shell systems when internalized in GL261 cells.