Non-covalent conjugate based on graphene oxide and a cytotoxic agent containing 1,3,5-triazine derivative and (5-phenyl-2H-tetrazol-2-yl)acetoxy moiety: Synthesis, characterization, properties and cytotoxic activity

The synthesis, identification and evaluation of the biological activity of a new non-covalent conjugate based on graphene oxide and (5-{[4,6-di(aziridin-1-yl)-1,3,5-triazin-2-yl]-amino}-2,2-dimethyl-1,3-dioxan-5-yl)methyl (5-phenyl-2H-tetrazol-2-yl)acetate were carried out. It was shown that the synthesized nanoconjugate is hemocompatible over the entire studied concentration range (10–100 μm) and demonstrates antiradical activity under irradiation. In addition, the conjugate exhibits cytotoxicity against A549 and HeLa cell lines with IC₅₀ values of 4.0 and 73.7 μm, respectively.